Comparisons of the diagnostic accuracies of optical coherence tomography, micro-computed tomography, and histology in periodontal disease: an ex vivo study

PURPOSE: Optical coherence tomography (OCT) is a noninvasive diagnostic technique that may be useful for both qualitative and quantitative analyses of the periodontium. Micro-computed tomography (micro-CT) is another noninvasive imaging technique capable of providing submicron spatial resolution. The purpose of this study was to present periodontal images obtained using ex vivo dental OCT and to compare OCT images with micro-CT images and histologic sections. METHODS: Images of ex vivo canine periodontal structures were obtained using OCT. Biologic depth measurements made using OCT were compared to measurements made on histologic sections prepared from the same sites. Visual comparisons were made among OCT, micro-CT, and histologic sections to evaluate whether anatomical details were accurately revealed by OCT. RESULTS: The periodontal tissue contour, gingival sulcus, and the presence of supragingival and subgingival calculus could be visualized using OCT. OCT was able to depict the surface topography of the dentogingival complex with higher resolution than micro-CT, but the imaging depth was typically limited to 1.2–1.5 mm. Biologic depth measurements made using OCT were a mean of 0.51 mm shallower than the histologic measurements. CONCLUSIONS: Dental OCT as used in this study was able to generate high-resolution, cross-sectional images of the superficial portions of periodontal structures. Improvements in imaging depth and the development of an intraoral sensor are likely to make OCT a useful technique for periodontal applications.

Detection of early changes in caries lesion using QLF-D and OCT / 대한구강보건학회지

OBJECTIVES: We aimed to compare the differences in caries lesion changes when measured by QLF-D as fluorescence loss and by SS-OCT as lesion depth with respect to demineralized time, during formation of artificial early caries lesion. We also demonstrated that QLF-D and SS-OCT can be used effectively in monitoring the longitudinal progression of simulated caries lesions. METHODS: Ten bovine incisors were sectioned (5x4 mm) and embedded in epoxy resin. An acid-resistant nail varnish was applied to a part of the tooth surfaces to protect sound enamel (2x4 mm). To generate lesions, each specimen was immersed in 40 ml of a demineralizing gel for 20 days at 37degrees C. To measure mineral loss of the demineralized specimens, fluorescence loss (DeltaF, %) was measured by QLF-D and lesion depth (microm) was determined by SS-OCT from the captured cross-sectional image. All the specimens were analyzed daily by QLF-D image analysis software and SS-OCT image analysis program for 20 days. The repeated measures analysis of DeltaF and lesion depth was used. The paired t-test was used to assess differences between each day. The correlation between DeltaF and lesion depth was determined using the Pearson's correlation coefficient. RESULTS: On the 5th, 10th, and 15th day, compared to baseline values, DeltaF decreased in 12.7%, 25.0%, and 33.6% of the specimens, respectively, and the lesion depth increased in 9.9%, 16.0%, and 22.6% of the specimens, respectively. However, after 15 days, there was no change in the DeltaF and lesion depth. High significant correlation was identified between the resultant values of DeltaF obtained by QLF-D and those of lesion depth obtained by SS-OCT (r = -0.811, P<0.0001). CONCLUSIONS: The QLF-D and SS-OCT could detect subtle changes in mineral loss and lesion depth with respect to demineralized time. Furthermore, these devices were useful for monitoring changes in mineral amount and lesion depth.